![]() ![]() Mardo K, Visnapuu T, Vija H, Aasamets A, Viigand K, Alamae T (2017) A highly active endo-levanase BT1760 of a dominant mammalian gut commensal Bacteroides thetaiotaomicron cleaves not only various bacterial levans, but also levan of Timothy Grass. With its ability to produce L-FOSs with specific chain lengths, LevBk could be attractively applied for converting of levan containing material to high value-added sweetener in the biorefinery industry. Based on enzymatic hydrolysis, short-chain L-FOSs with degree of polymerization (DP) of 2–4 were produced from hydrolysis of timothy grass levan under optimal conditions for 9–24 h. LevBk optimally worked at 45 ☌, pH 6.0 with the specific activity of 2.43 U/mg. Based on sequence and structural analysis, LevBk was classified as a member of endo-levanase in GH32 family containing N-terminal substrate binding pocket and C-terminal β-sandwich domains. Soluble LevBk protein was produced in Escherichia coli BL21(DE3) system at 40 mg/L of culture medium. In this work, a recombinant levanase (LevBk) from Bacillus koreensis strain HL12 was characterized. Endo-levanase, which randomly hydrolyzes β-(2,6)-linkages in fructans, is a promising enzyme for short-chain FOS production. Levan-type fructooligosaccharides (L-FOSs) are a prominent class of non-digestible oligosaccharides with potential as nutritional prebiotics. ![]()
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